The suburb has a peak-hour ambulance service that runs from 9am-9pm.

The Craigieburn and District First Response Team, a volunteer organisation, responds to medical emergencies and after-hours calls.

Craigieburn Residents Association secretary Brenda Davies says residents are forced to wait more than 20 minutes to be transferred to a hospital because the First Response Team cannot transfer patients.

"The team does a great job, but when a person has a heart attack it's important they get to a hospital as soon as possible," Ms Davies said.

"The ambulance can sometimes come from fairly far out so it takes longer for them to get here."

Ms Davies said the residents association had written a number of letters to the state government requesting a 24-hour service be offered immediately.

"Now is the time to do it because more and more people are coming into the area, which means the chance of more accidents at home or on the roads," she said.

Ambulance Employees Australia state secretary Steve McGhie said a 24-hour ambulance service should have been introduced in the suburb years ago.

"Craigieburn is one of the fastest-growing areas in the state and I'm disappointed the state government hasn't responded to our submissions in the past to put a 24-hour service in the area," Mr McGhie said.

"Craigieburn already has the infrastructure for the service and all it needs is an extra four staff members."

A spokeswoman for Health Minister Daniel Andrews said: "The government recognises the valuable role the First Response Team plays in providing an early response to acute patients across the Craigieburn community. Ambulance Victoria has advised it's in regular contact with the response team volunteers and monitoring the sustainability of caseload and rostering arrangements."